The Belt and Road Initiative (B&R Initiative) is a crucial strategy to promote regional sustainable development in the new era. However, the realization of the B&R Initiative faces huge challenges because of the dual characteristics of a fragile eco-environment and strong dependence on ecological resources for economic development in the Belt and Road (B&R) countries. The ecological carrying capacity (ECC) is a crucial indicator for evaluating regional sustainable development. From the perspective of the relationship between the supply and consumption of ecological resources, this study uses net primary productivity data to measure the supply capacity of ecological resources, and it uses the agricultural production and trade data provided by the United Nations Food and Agriculture Organization to measure the level of ecological resource consumption. These supply and consumption data are then used to assess the ECC and ecological carrying status (ECS) of the B&R countries in 2017. The results show that: (1) The ECC of the B&R is 11.097 billion people; the ecosystem can also support 6.433 billion people, and the ECC is in a state of rich and surplus. (2) The ECS is polarized among the regions and countries along the B&R. Of the 65 countries, the ECC of 40 countries is in a rich and surplus state, mainly in Mid-East Europe and Southeast Asia, while the ECC of 19 countries is in severe overload, mainly in West Asia/Middle East. (3) Although the ecosystems still have ample carrying space in countries along the B&R, ecological protection is still facing enormous challenges during the implementation of the B&R Initiative combined with the internationally recognized ecological protection standards as well as the forecasts of the population and economic development. As the core content of building a new international trade network, the B&R Initiative will help to solve the spatial mismatch between the supply and consumption of ecological resources, which provides a new opportunity to coordinate the contradiction between the ecological protection and social demands of the B&R countries.

Key Biodiversity Areas (KBAs) are ecological conservation priorities proposed by IUCN and widely recognized by most countries. Evaluating the changes in the ecological characteristics in KBAs is important for biodiversity conservation and the construction of Protected Areas (PAs). There are various ecosystem types in the Belt and Road Initiative (BRI) region, which has an extremely high value of biodiversity conservation, and the KBAs should be the prime targets of ecological protection efforts. Using the data of land cover, NDVI and Nighttime Light (NTL), we analyzed the ecological conditions of the KBAs in the BRI region, and their temporal and spatial variations, from the perspectives of vegetation coverage and human activities. The conclusions are: (1) There is generally no significant difference in the land cover of the KBAs, among which forest, wilderness and grassland are the main types; (2) The NDVI of the KBAs showed an increase, indicating that the vegetation was gradually improving, while a few KBAs presenting vegetation degradation were mainly distributed in the Indochina Peninsula, Qinghai-Tibet Plateau and Central and Western Asia; and (3) The NTL in the KBAs was very low, indicating that the human pressure on the natural ecosystems was limited, and only a few KBAs distributed in Central and Eastern Europe, India, and the Indochina Peninsula have high human activity intensity which also showed an increase. This study emphasizes that we should make full use of the biome succession law, and limit the interference of human activities on natural ecosystems for ecological protection of the KBAs, so as to continuously make new breakthroughs in the construction of Protected Areas (PA) in the BRI region.