Spatial Analysis and Biogeochemical Cycles: A Comparative Study of Kashin-Beck Disease Villages and Non-disease Villages in Linzhou County, Tibet

  • 1. Lhasa National Ecological Research Station, Key Laboratory of Ecosystem Network Observation and Modelling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China;
    2. University of Chinese Academy of Sciences, Beijing 100049, China;
    3. State Key Laboratory of Resources and Environmental Information System, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China

Received date: 2019-10-12

  Accepted date: 2020-01-10

  Online published: 2020-05-30

Supported by

The Key Research and Development and Transformation Program of Tibet (XZ201901NB08); The Major Science and Technology Project of Tibet (XZ201901NA03, XZ201801NA02).


This study investigated Linzhou County in Tibet, which currently hosts the most serious outbreak of Kashin-Beck disease (KBD) in China. This study uses the geographical detector (GeoDetector) algorithm to measure the influences that several risk factors have on KBD prevalence and validates the spatial analysis results with environmental chemistry. Based on a comprehensive examination of 10 potentially related spatial factors and an environmental chemistry analysis of the soil-water-grain-human biogeochemical cycle in the local KBD and non-KBD villages, four main conclusions are drawn. (1) KBD in Linzhou County is a consequence of multiple interrelated environmental factors, of which the most important controlling factor is the stratum factor. (2) The concentrations of selenium (Se) in all environmental media (soil, water, and food) and human tissue in the KBD villages in Linzhou County are lower than those of the non-KBD villages. (3) The intake of Se and chromium (Cr) by local residents is seriously insufficient, especially the average daily dose by ingestion (ADD) for Se in the KBD village, which is only about 4% of the World Health Organization (WHO) recommended lower limit for adult elemental intake. (4) We speculate that the main cause for the local KBD outbreak is a lack of Se in the stratum. This absence leads to a serious Se deficiency in the local population through ecosystem migration and transformation, which will eventually lead to an endemic biogeochemical Se deficiency.

Cite this article

TIAN Yuan, ZHA Xinjie, GAO Xing, DAI Erfu, YU Chengqun . Spatial Analysis and Biogeochemical Cycles: A Comparative Study of Kashin-Beck Disease Villages and Non-disease Villages in Linzhou County, Tibet[J]. Journal of Resources and Ecology, 2020 , 11(2) : 232 -246 . DOI: 10.5814/j.issn.1674-764x.2020.02.011


1 Aguiar A R, Saiki M.2001. Determination of trace elements in human nail clippings by neutron activation analysis.Journal of Radioanalytical & Nuclear Chemistry, 249(2): 413-416.
2 AQSIQ and SAC (General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China, Standardization Administration of the People’s Republic of China). 2008. Drinking natural mineral water (GB 8537-2008). Beijing: China Standards Press. (in Chinese)
3 Basangzhuoma, Yangla, Ciyang, et al.2012. Epidemiological investigation on Kashin-Beck Disease pedigrees in Tibet.Journal of Tibet University (Natural Science Edition), 27(2): 47-51. (in Chinese)
4 Chasseur C, Begaux F, Suetens C, et al.2002. Is Kashin-Beck disease related to the presence of fungi on grains? In: Proceedings of the 8th international working conference on stored product protection, Kalinović I and Rozman V (ed.), York, UK.
5 Chen L, Wang G C, Hu F S, et al.2014. Groundwater hydrochemistry and isotope geochemistry in the Turpan Basin, northwestern China.Journal of Arid Land, 6(4): 378-388.
6 Cheng S K, Min Q W.2002. Strategies of agriculture and animal husbandry development in Tibet autonomous region.Resources Science, 24(5): 1-7. (in Chinese)
7 Chidambaram S, Anandhan P, Prasanna M V, et al.2013. Major ion chemistry and identification of hydrogeochemical processes controlling groundwater in and around Neyveli Lignite Mines, Tamil Nadu, South India.Arabian Journal of Geosciences, 6(9): 3451-3467.
8 CNS (Chinese Nutrition Society). 2014. Chinese dietary reference intakes handbook (2013 edition). Beijing: China Standard Press. (in Chinese)
9 EPA (Environmental Protection Agency of United States). 1993. Determination of inorganic anions by ion chromatography. Cincinnati, Ohio: Environmental Protection Agency Environmental Monitoring Systems Laboratory Office of Research and Decelopment.
10 EPA (Environmental Protection Agency of United States). 2001. Risk Assessment Guidance for Superfund Volume I: Human Health Evaluation Manual (Part D, Standardized Planning, Reporting, and Review of Superfund Risk Assessments). Washington DC: Office of Emergency and Remedial Response U.S. Environmental Protection Agency.
11 EPA (Environmental Protection Agency of United States). 2004. Risk Assessment Guidance for Superfund Volume I: Human Health Evaluation Manual (Part E, Supplemental Guidance for Dermal Risk Assessment). Washington DC: Office of Superfund Remediation and Technology Innovation U.S. Environmental Protection Agency.
12 FAO, WHA (Food and Agriculture Organization of the United Nations, World Health Organization). 2002. Human vitamin and mineral requirements, paper presented at Report of a joint FAO/WHO expert consultation, Bangkok, Thailand, 21-30 Sep. 1998.
13 FAO, WHA (Food and Agriculture Organization of the United Nations, World Health Organization). 2013. Vitamin and mineral requirements in human nutrition, Second ed.: 362.
14 Fordyce F M.2013. Selenium deficiency and toxicity in the environment. In: Selinus O (edited). Essentials of medical geology, Netherlands: Springer: 375-416.
15 Gibbs R J.1970. Mechanisms controlling world water chemistry.Science, 170(3962): 1088-1090.
16 Grange M L, Mathieu F, Begaux F, et al.2001. Kashin-Beck disease and drinking water in Central Tibet.International Orthopaedics, 25(3): 167-169.
17 Guo X.2001. Diagnostic, clinical and radiological characteristics of Kashin-Beck disease in Shaanxi Province, PR China.International Orthopaedics, 25(3): 147-150.
18 Guo X.2008. Progression and prospect of etiology and pathogenesis of Kashin-Beck disease.Journal of Xi’an Jiaotong University (Medical sciences), 29(5): 481-488. (in Chinese)
19 Guo X, Ning Y J, Wang X.2015. Chapter 31: Selenium and Kashin-Beck disease. Cambridge, UK: Royal Society of Chemistry.
20 Höllriegl V, Wei B L, Oeh U.2006. Human biokinetics of strontium—part II: Final data evaluation of intestinal absorption and urinary excretion of strontium in human subjects after stable tracer administration.Radiation & Environmental Biophysics, 45(3): 179-185.
21 Hinsenkamp M, Mathieu F, Claus W, et al.2009. Effects of physical environment on the evolution of Kashin-Beck disease in Tibet.International Orthopaedics, 33(4): 1085-1088.
22 Hopps H C.1977. The biologic bases for using hair and nail for analyses of trace elements.Science of the Total Environment, 7(1): 71-89.
23 Hutchinson M F, Xu T B.2013. Anusplin. Canberra, Australia: The Australian National University.
24 Konikowska K, Mandecka A.2018. Trace elements in human nutrition. London, UK: Wiley-Blackwell.
25 Li S H, Xiao T F, Zheng B S.2012. Medical geology of arsenic, selenium and thallium in China.Science of the Total Environment, 422(1): 31-40.
26 Li S J, Li W, Hu X, et al.2009. Soil selenium concentration and Kashin-Beck disease prevalence in Tibet, China.Frontiers of Environmental Science & Engineering, 3(1): 62-68.
27 Lintas C, Mariani-Costantini A.1991. The Mediterranean diets in health and disease, Chapters 3: Cereal foods: Wheat, corn, rice, barley, and other cereals and their products. Boston: Springer: 316.
28 Mahmoodi M M, Mohammad R, Yi Z, et al.1998. Kashin-Beck osteoarthropathy in rural Tibet in relation to selenium and iodine status.New England Journal of Medicine, 339(16): 1112-1120.
29 Malaisse F, Mathieu F.2008. Big bone disease. Belgium: Agro de Gembloux: 85-100.
30 MEE (Ministry of Ecology and Environment of the People’s Republic of China). 1990. The element background values of Chinese soil. Beijing: China Environmental Science Press: 501. (in Chinese)
31 MEE (Ministry of Ecology and Environment of the People’s Republic of China). 2018. Soil environmental quality (GB 15618-2018), in Risk control standard for soil contamination of agricultural land. Beijing: China Standards Press. (in Chinese)
32 MEE (Ministry of Ecology and Environment of the People’s Republic of China). 2002. Methods for chemical analysis of water and waste water. Beijing: China Environmental Science Press. (in Chinese)
33 Ministry of Health of the People’s Republic of China. 2007a. Standards examination methods for drinking water (GB/T 5750-2006). Beijing: China Standards Press.
34 MH (Ministry of Health of the People’s Republic of China). 2007b. Standards for drinking water quality (GB 5749-2006). Beijing: China Standards Press. (in Chinese)
35 MA (Ministry of Agriculture of the People’s Republic of China). 2005. Limits of eight elements in cereals, legume, tubes and its products (NY 861-2004). Beijing: China Standards Press. (in Chinese)
36 National Kashin-Beck Disease Surveillance Group, Chinese Center for Disease Control and Prevention.2002. The monitoring report of Kashin-Beck disease prevalence rate of whole country in 2002.Chinese Journal of Endemiology, 21(5): 368-370. (in Chinese)
37 Pasteels J L, Liu F D, Hinsenkamp M, et al.2001. Histology of Kashin-Beck lesions.International Orthopaedics, 25(3): 151-153.
38 Peng A, Yang C, Rui H, et al.1992. Study on the pathogenic factors of Kashin-Beck disease.Journal of Toxicology & Environmental Health, 35(2): 79-90.
39 Peng A, Wang W H. Wang C X.1999. The role of humic substances in drinking water in Kashin-Beck disease in China.Environmental Health Perspectives, 107(4): 293-296.
40 Piper A M.1944. A graphic procedure in the geochemical interpretation of water analyses.Transactions American Geophysical Union, 25(1): 27-39.
41 Qi Y G, Ming X Y.1995. Studies on human dietary requirements and safe range of dietary intakes of selenium in China and their application in the prevention of related endemic diseases.Biomedical & Environmental Sciences, 8(3): 187-201.
42 Rayman M P.2000. The importance of selenium to human health.The lancet, 356(9225): 233-241.
43 Roman M, Jitaru P, Barbante C.2013. Selenium biochemistry and its role for human health.Metallomics, 6(1): 25-54.
44 Schroeder A H, Frost D V, Balassa J J.1970. Essential trace metals in man: Selenium.Journal of Chronic Diseases, 23(4): 227-243.
45 Seale L A, Berry M J.2013. Selenium in human health and disease.Antioxidants & Redox Signaling, 14(7): 1337-1383.
46 Stone R.2009. Diseases: A medical mystery in middle China.Science, 324(5933): 1378-1381.
47 Sun L Y, Li Q, Meng F G, et al.2012. T-2 toxin contamination in grains and selenium concentration in drinking water and grains in Kaschin-Beck disease endemic areas of Qinghai Province.Biological Trace Element Research, 150(1): 371-375.
48 Suttle N F.2010. Mineral nutrition of livestock, 4th ed. UK: The Centre for Agriculture and Bioscience International (CABI): 596.
49 Takagi Y, Matsuda S, Imai S, et al.1986. Trace elements in human hair: An international comparison.Bulletin of Environmental Contamination & Toxicology, 36(1): 793-800.
50 Tan J A.1989. The atlas of endemic diseases and their environments in the People’s Republic of China. Beijing: Science Press. (in Chinese)
51 Tan J A, Zhu W Y, Wang W Y, et al.2002. Selenium in soil and endemic diseases in China.Science of the Total Environment, 284(1-3): 227-235.
52 Tang H J, Li Z M.2012. Study on per capita grain demand based on Chinese reasonable dietary pattern.Scientia Agricultura Sinica, 45(11): 2315-2327. (in Chinese)
53 Tian Y, Yu C Q, Zha X J, et al.2016. Distribution and potential health risks of Arsenic, Selenium, and Fluorine in natural waters in Tibet, China.Water, 8(12): 568. DOI: 10.3390/w8120568.
54 Tibet Kaschin-Beck Disease Study Group, Chinese Center for Disease Control and Prevention.2000. Investigative report on the prevalence condition of Kashin Beck Diserase (KBD) in Tibet.Chinese Journal of Cndemiology, 19(1): 41-43. (in Chinese)
55 Tobler W R.1970. A computer movie simulating urban growth in the detroit Region.Economic Geography, 46(S1): 234-240.
56 Uchida S, Tagami K, Hirai I.2007. Soil-to-plant transfer factors of stable elements and naturally occurring radionuclides (1) upland field crops collected in Japan AU-UCHIDA, Shigeo.Journal of Nuclear Science and Technology, 44(4): 628-640.
57 Underwood E J.1956. Trace elements in human and animal nutrition. New York: Academic Press.
58 Wang J F, Hu Y.2012. Environmental health risk detection with GeoDetector.Environmental Modelling & Software, 33(10): 114-115.
59 Wang J F, Xu C D.2017. Geodetector: Principle and prospective.Acta Geographica Sinica, 72(1): 116-134. (in Chinese)
60 Wang J F, Zhang T L, Fu B J.2016. A measure of spatial stratified heterogeneity.Ecological Indicators, 67: 250-256.
61 Wang J F, Li X H, Christakos G, et al.2010. Geographical detectors-based health risk assessment and its application in the neural tube defects study of the Heshun Region, China.International Journal of Geographical Information Science, 24(1): 107-127.
62 Wang J, Li H R, Li Y H, et al.2013. Speciation, distribution, and bioavailability of soil selenium in the Tibetan Plateau Kashin-beck disease area--A case study in Songpan County, Sichuan Province, China.Biological Trace Element Research, 156(1-3): 367-375.
63 Wang J, Li H R, Yang L S, et al.2017. Distribution and translocation of selenium from soil to highland barley in the Tibetan Plateau Kashin-Beck disease area.Environmental Geochemistry & Health, 39(1): 221-229.
64 Watson W S, Morrison J, Bethel M I, et al.1986. Food iron and lead absorption in humans.American Journal of Clinical Nutrition, 44(2): 248-256.
65 WHO (World Health Organization). 1996. Trace elements in human nutrition and health. Geneva, Switzerland.
66 WHO (World Health Organization). 2008. Guidelines for drinking-water quality. Geneva, Switzerland.
67 Yamamuro T.2001. Kashin-Beck disease: A historical overview.International Orthopaedics, 25(3): 134-137.
68 Yang C L, Niu C C, Bodo M, et al.1993. Fulvic acid supplementation and selenium deficiency disturb the structural integrity of mouse skeletal tissue: An animal model to study the molecular defects of Kashin-Beck disease.Biochemical Journal, 289(3): 829-835.
69 Yang L S, Lv Y, Li H R, et al.2006. Features of geographical environment of Kaschin-Beck Disease (KBD) affected region in Tibet.Scientia Geographica Sinica, 26(4): 466-471. (in Chinese)
70 Zha X J, Gao X.2019. Ecological analysis of Kashin-Beck osteoarthropathy risk factors in Tibet’s Qamdo City, China.Scientific Reports, 9(1): 2471. DOI: 10.1038/s41598-019-39792-0.
71 Zhang B J, Yang L S, Wang W Y, et al.2011. Environmental selenium in the Kaschin-Beck disease area, Tibetan Plateau, China.Environmental Geochemistry & Health, 33(5): 495-501.
72 Zhao Z J, Li Q, Yang P Z, et al.2013. Selenium: A protective factor for Kaschin-Beck disease in Qing-Tibet Plateau.Biological Trace Element Research, 153(1-3): 1-4.