Karst Ecosystem

The Ecological Importance and Influence of Land Use on Carbonic Anhydrase Activity of Waters in the Upstream Section of the Wujiang River Basin

  • 1 Institute of Resource Biology and Biotechnology, Department of Biotechnology, College of Life Science & Technology, Huazhong University of Science & Technology, Wuhan 430074, China;
    2 Institute of Karst Geology, Chinese Academy of Geological Sciences, Guilin 541004, China;
    3 Key Laboratory of Karst Dynamics, Ministry of Land and Resources/Guangxi Zhuang Autonomous Region, Guilin 541004, China;
    4 Teachers Service Commission-Kenya, P.O. Box Private Bag 00100 Nairobi, Kenya

Online published: 2015-07-30


Thirty-four sampling sites for surface water and twenty-seven sampling sites for ground water in areas of different land use were selected in the Wujiang River Basin, upstream section in Guizhou Province, China. Carbonic anhydrase (CA) activity in surface and ground water was analyzed and compared. The results show that CA activity was detected in all surface and ground water samples. In general, average CA activity in surface and ground water was highest from samples in arbor lands, followed by shrub lands, shrub and grass lands, agricultural lands, and residential areas. The average CA activity in ground water from arbor lands, shrub lands, and shrub and grass lands was higher than that in surface water. Water CA activity was positively correlated with HCO3concentration in surface water and ground water. These results imply that the contribution of CA in water to the karst carbon sink potential of river basins warrants further extensive and in-depth research.

Cite this article

PAN Weizhi, SILA Onesmus Nzung’a, LI Wei, HUANG Qibo, SHEN Taiming, WANG Chenwei, QIN Xiaoqun and YU Longjiang . The Ecological Importance and Influence of Land Use on Carbonic Anhydrase Activity of Waters in the Upstream Section of the Wujiang River Basin[J]. Journal of Resources and Ecology, 2015 , 6(4) : 230 -236 . DOI: 10.5814/j.issn.1674-764x.2015.04.006


Cao J H, Yang H, Kang Z Q, 2011. Preliminary regional estimation of carbon sink flux by carbonate rock corrosion: A case study of the Pearl River Basin. Chinese Science Bulletin, 56(35): 3766-3773.
Elleuche S, S P?ggeler. 2010. Carbonic anhydrases in fungi. Microbiology, 156: 23-29.
Favre N, M L Christ, A C Pierre. 2009. Biocatalytic capture of CO2 with carbonic anhydrase and its transformation to solid carbonate. Journal of Molecular Catalysis B: Enzymatic, 60(3): 163-170.
Guo Y N, Feng X B, Li Z G, et al. 2008. Distribution and wet deposition fluxes of total and methyl mercury in Wujiang River Basin, Guizhou, China. Atmospheric Environment, 42: 7096-7103.
Jiang Z C, Yuan D X. 1999. CO2 source-sink in karst processes in karst areas of China. Episodes, 22(1): 33-35.
Johnson M J, K Y Lee, K M Scow. 2003. DNA fingerprinting reveals links among agricultural crops, soil properties, and the composition of soil. Geoderma, 114(3-4): 279-303.
Lasco R D, J S Lales, M T Arnuevo, et al. 2002. Carbon dioxide (CO2) storage and sequestration of land cover in the Leyte Geothermal Reservation. Renewable Energy, 25(2): 307-315.
Lian B, Yuan D X, Liu Z H. 2011. Effect of microbes on karstification in karst ecosystems. Chinese Science Bulletin, 56: 3743-3747.
Liu Y, Liu Z H, Zhang J L, et al. 2010. Experimental study on the utilization of DIC by Oocystis solitaria Wittr and its influence on the precipitation of calcium carbonate in karst and non-karst waters. Carbonates Evaporites,
25: 21-26.
Liu Z H, Dreybrodt W. 1997. Dissolution kinetics of calcium carbonate minerals in H2O-CO2 solutions in turbulent flow: The role of the diffusion boundary layer and the slow reaction CO2+H2O ? HCO3 +H+. Geochimica et Cosmochimica Acta, 61(14): 2879-2889.
Liu Z H, W Dreybrodt, Wang H J. 2010a. A new direction in effective accounting for the atmospheric CO2 budget: Considering the combined action of carbonate dissolution, the global water cycle and photosynthetic uptake of DIC by aquatic organisms. Earth-Science Reviews, 99(3): 162172.
Li W, Chen W S, Zhou P P, Yu L J. 2013. Influence of enzyme concentration on bio-sequestration of CO2 in carbonate form using bacterial carbonic anhydrase. Chemical Engineering Journal, 232: 149-156.
Li W, Liu L P, Chen W S, et al. 2010. Calcium carbonate precipitation and crystal morphology induced by microbial carbonic anhydrase and other biological factors. Process Biochemistry, 45: 1017-1021.
Li W, Yu L J, He Q F, et al. 2005a. Effects of microbes and their carbonic ++ anhydrase on Ca2 and Mg2 migration in column-built leached soil-limestone karst systems. Applied Soil Ecology, 29(3): 274-281.
Li W, Yu L J, Wu Y, et al. 2007. Enhancement of Ca2 release from limestone by extracellular carbonic anhydrase. Bioresour Technology, 98: 950-953.
Li W, Yu L J, Yuan D X, et al. 2005b. A study of the activity and ecological significance of carbonic anhydrase from soil and its microbes from different karst ecosystems of Southwest China. Plant and Soil, 272: 133141.
Li W, Zhou P P, Jia L P, et al. 2009. Limestone dissolution induced by fungal mycelia, acidic materials, and carbonic anhydrase from fungi. Mycopathologia, 167(1): 37-46.
Sala O E, III F S Chapin, J J Armesto, et al. 2000. Global biodiversity scenarios for the year 2100. Science, 5459(287): 1770-1774.
Shen T M, Li W, Zhang Q, et al. 2012. Carbonic anhydrase activity of the water-body in different eco-environments of river basins: A case study in the Guijiang river basin. Carsologica Sinica, 31(4): 409-414.
Smith K S, J G Ferry. 2000. Prokaryotic carbonic anhydrases. FEMS Microbiology Reviews, 24: 335-366.
Steenwerth K L, L E Jackson, F J Calderón, et al. 2002. Soil microbial community composition and land use history in cultivated and grassland ecosystems of coastal California. Soil Biology and Biochemistry, 34(11): 1599-1611.
Yadav R R, K Krishnamurthi, S N Mudliar, et al. 2014. Carbonic anhydrase mediated carbon dioxide sequestration: Promises, challenges and future prospects. Journal of Basic Microbiology, 54: 472-481.
Zhang C. 2011. Carbonate rock dissolution rates in different land uses and their carbon sink effect. Chinese Science Bulletin, 56(35): 3759-3765.
Zhu J, Wang Y, Liu C, Tao F. 2005. A preliminary study on the distribution characteristics of nutrients (N, P, Si, C) in the Wujiang River Basin. Chinese Journal of Geochemistry, 24(4): 352-360.
Zolotov Y A, V M Ivanov, V G Amelin. 2002. Test methods for extra-laboratory analysis. Trends in Analytical Chemistry, 21(4): 302-319.