Fluorescence Properties of Submerged Macrophytes in Nanjishan Wetland, Southern Poyang Lake

  • 1 Key Laboratory of Poyang Lake Wetland and Watershed Research, Ministry of Education, Jiangxi Normal University, Nanchang 330022, China;
    2 College of Life Science, Jiangxi Normal University, Jiangxi Provincial Key Lab of Protection and Utilization of Subtropical Plant Resources, Nanchang 330022, China;
    3 Jiangxi Nanjishan Wetland National Nature Reserve Bureau, Nanchang 330009, China

Received date: 2014-06-04

  Revised date: 2014-11-28

  Online published: 2015-01-18

Supported by

National Natural Science Foundation of China (41161035, 41461042); National Science and Technology Supporting Program of China (2011BAC13B02); Collaborative Innovation Center for Major Ecological Security Issues of Jiangxi Province and Monitoring Implementation (No. JXS-EW-03); and Jiangxi Province Science and Technology Support Program (20133BBG70005).


We used a FluorCam portable chlorophyll fluorescence imaging system to measure QY-max (the maximum light quantum yield, Fv/Fm, the largest light quantum efficiency of PS II) of submerged plants in wetlands of Baisha Lake and Changhu Lake, Jiangxi Nanjishan Wetland National Nature Reserve, in winter 2013. Specifically, we measured ΦPS II (PS II actual quantum efficiency), qP (photochemical quenching) and corresponding fluorescence images. Using the visual method and sampling sites method to obtain coverage, richness and abundance of submerged plants, and determined nutrient levels in water. The results show that the QY-max of Hydrilla verticillata and Vallisneria natans in Baisha Lake ranged from 0.48 to 0.68 and 0.52 to 0.71, respectively; the ΦPS II of these two species ranged from 0.32 to 0.58 and 0.20 to 0.46, respectively. The two plants had similar photosynthetic efficiency. The QY-max of Nymphoides peltatum and V. natans in Changhu Lake ranged from 0.66 to 0.77 and 0.19 to 0.68, respectively; the ΦPS II of these two species ranged from 0.26 to 0.48 and 0.22 to 0.43, respectively. The observed higher photosynthetic efficiency of N peltatum suggests it is more likely to become the dominant species. In Baisha Lake, the frequency of occurrence of plants was: H. verticillata, 90%; V. natans, 93.3%; Najas minor 26.7%, and Potamogeton franchetii 10%. In Changhu Lake, the frequency of N. peltatum was 86.7%, V. natans was 16.7%, and N. minor was 56.7%. The overall frequency of submerged plants living in Baisha Lake was much higher than that of submerged plants living in Changhu Lake, with different species dominating the two lakes. According to comprehensive analysis and comparison of trophic levels, biodiversity and photosynthetic fluorescence characteristics in the two lakes, eutrophication of Baisha Lake was higher than for Changhu Lake; and H. verticillata and V. natans were the dominant species, with similar photosynthetic activity. Conversely, in Changhu Lake, N. peltatum and V. natans were the dominant species, but the photosynthetic activity of N. peltatum was higher than V. natans. Differences in eutrophication levels in different water bodies in the Nanjishan Wetland and differing ecological niches of submerged plant species are characteristic of this system.

Cite this article

JIAN Minfei, WANG Sichen, YU Houping, LI Lingyu, JIAN Meifeng, YU Guanjun . Fluorescence Properties of Submerged Macrophytes in Nanjishan Wetland, Southern Poyang Lake[J]. Journal of Resources and Ecology, 2015 , 6(1) : 52 -59 . DOI: 10.5814/j.issn.1674-764x.2015.01.007


Chen J M, Yu X P, Cheng J A. 2006. The application of chlorophyll fluorescence kinetics in the study of physiological responses of plants to environmental stresses. Acta Agriculturae Zhejiangensis, 18(1): 51-55. (in Chinese)
Chen M, Tang Y L. 2013. Chlorophyll fluorescence characteristics of amaranth under heat stress. Chinese Journal of Ecology, 32(7):1813-1818. (in Chinese)
Chen Y, Zhao Y Y, Pan S Y, et al. 2009. Classification determination of Phyto- PAM on chlorophyll in phytoplankton. Modern Scientific Instruments, (4): 100-103 (in Chinese)
Chu J Z. 2009. Study on physiological effects of Sediment and water eutrophication status on submerged plant. Baoding: Agricultural University of Hebei. (in Chinese)
Fang J Y, Wang X P, Shen Z H, et al. 2009. Methods and protocols for plant community inventory. Biodiversity Science, 17(6): 533-548. (in Chinese)
Fang Y Y, Yang X E, Chang H Q, et al. 2008. In situ remediation of polluted water body by planting hydrophytes. Chinese Journal of Applied Ecology, 19(2): 407-412. (in Chinese)
Govindjee, L Nedbal. 2000. Seeing is believing. Photosynthetica, 38: 481- 482.
Hu X H, Pu G L, Xiao Q W, et al. 2007. Study on chlorophyll fluorescence characteristics of plum trees under water stress. Chinese Journal of Eco-Agriculture, 15(1): 95-77. (in Chinese)
Kausky H, A Hirsch. 1931. Neue versuche zur kohlens ure-assimilation. Naturwissenschaften, 19: 64. (in Geman)
Kim J-H, Jung J-E, Lee C-H. 2002. In vivo monitoring of the incorporation of chemicals into cucumber and rice leaves by chlorophyll fluorescence imaging. Journal of Plant Biotechnology, 4(4): 173-179.
Kuang Q J, Xia Y Z, Wu Z B. 1997. Research artificial simulation ecosystem of aquatic plants and algae. Journal of Artificial Simulation of Aquatic Organisms, 21(1): 90-94 (in Chinese).
Lacoul P, B Freedman. 2006. Relationships between aquatic plants and environmental factors along a steep Himalayan altitudinal gradient. Aquatic Botany, 84:3-16.
Li D M, Yang Z, Yu Y. 2013a. Study on photosynthetic activity of phytoplankton in spring in Ge Lake and Luoma Lake. Lake Sciences, 25(5): 688-694. (in Chinese)
Li D M, Yang Z, Yu Y. 2013b. Photochemical vitality of cyanobacteria in Lake Taihu in spring and autumn season. Acta Scientiae Circumstantiae, 33(11): 3053-3059, (in Chinese)
Li E H. 2006. Effects of aquatic macrophytes on nutrient cycling in shallow freshwater lake ecosystems. Wuhan: Institute of Hydrobiology, Chinese Academy of Sciences. (in Chinese)
Lichtenthaler H K, F Babani, G Langsdorf, et al. 2000. Measurement of differences in red chlorophyll florescence and photosynthetic activity between sun and shade leaves by fluorescence imaging. Photosynthetica, 38(4): 521-529.
Liu J K. 1999. Senior aquatic biology. Beijing: Science Press. (in Chinese). Liu X Z, Fan S B, Hu B H. 2005. Comprehensive and scientific survey of Jiangxi Nanjishan Wetland Nature Reserve. Beijing: China Forestry Press, 11. (in Chinese)
Liu Y, Wang S R, Jin X C, et al. 2009. Effect of water nutrient levels on growth and antioxidant enzyme activities of 3 kinds of submerged plants. Chinese Journal of Ecology, 18(1):57-63. (in Chinese)
Omasa K, Shimasaki K I, Aiga I, et al. 1987. Image analysis of chlorophyll fluorescence transients for diagnosing the photosynthetic system of attached leaves. Plant Physiology, 84: 748-752.
Preston C D. 1996. Aquatic and wetland plants of India. New York: Oxford University Press.
Qin B Q, Gao G, Hu W P, et al. 2005. Reflections on the theory and practice of shallow lake ecosystem restoration. Journal of Lake Sciences, 17(1): 9-16. (in Chinese)
Scholes J D, S R Rolfe. 1996. Photosynthesis in localised regions of oat leaves infected with crown rust (Puccinia coronata): Quantitative imaging of chlorophyll fluorescence. Planta, 199: 573-582.
Song Y Z, Cai W, Qing B Q. 2009. Photosynthetic fluorescence properties of common floating leaf plant and submerged plant in Tai lake. Journal of Applied Ecology, 20(3): 569-573. (in Chinese).
Wang G X, Cheng X Y, Pu P M. 2002. Lake eutrophication control in technology, theory and application. Journal of Lake Sciences, 14(3): 273- 282. (in Chinese)
Wang W J, He H S, Guan Y. 2009. Comparison of acetone and DMSO method for determination of chlorophyll and carotenoids contents. Plant Research, 29(2): 224-229. (in Chinese).
Wei F S. 2002. Standard methods for the analysis of water and wastewater. 4th Ed. Beijing: China Environmental Science Press, 649-671. (in Chinese)
Woolhouse H W. 1974. Longevity and senescence in plant. Science Progress, 61: 123-147.
Yang D D, Xiong J L. 2006. Investigation and evaluation of reptiles and amphibians resources of nature reserve in Nanjishan in Jiangxi. Sichuan Animal, 25(2): 285-289. (in Chinese)
Ye J Y. 1985. The A rnon calculation formula of the determination of chlorophyll content. Plant Physiology Communications, (6): 69. (in Chinese)
You X, Gong J R. 2012. Significance and application of chlorophyll fluorescence dynamics process parameters. Journal of West China Forestry Science, 41(5): 90-94. (in Chinese)
Yuan J, Cui G F, Lei T. 2009. Main environmental factors effect on composition of the submerged macrophytes population in wetland in Beijing. Chinese Journal of Ecology, 28(11): 2189-2196. (in Chinese)
Zhang J P, Huang X P. 2009. Application of ecology of seagrass in the chlorophyll fluorescence technique. Marine Environment Science, 28(6): 772-777. (in Chinese)