Comparative Study of the Impact of Drought Stress on P.centrasiaticum at the Seedling Stage in Tibet

doi:10.5814/j.issn.1674-764X.2020.03.010

Abstract

Abstract:

Pennisetum centrasiaticum is widely distributed in arid and semi-arid areas of Tibet. Its rhizome system is developed and has strong resistance to adversity. In this study, the physiological characteristics and drought resistance of P.centrasiaticum seedlings from 12 drought-stressed sites in Tibet were examined at the Lhasa Plateau Ecosystem Research Station of the Chinese Academy of Sciences. PEG-6000 solution with five levels of water potential (0, -0.7, -1.4, -2.1, and -2.8 MPa) was used to simulate drought stress, and malondialdehyde (MDA), proline (Pro) and chlorophyll contents were determined. The balance between production and elimination of reactive oxygen species in P.centrasiaticum was destroyed, leading to membrane lipid peroxidation and the production of MDA, and accelerating the decomposition of chlorophyll. P.centrasiaticum absorbed water from the outside to resist drought by secreting proline and other osmotic regulating substances. The Pro and chlorophyll contents in P.centrasiaticum showed a temporary rising trend, and then decreased with the decrease in water potential. MDA content increased with the decrease in water potential. By using the membership function method, the drought resistance of P.centrasiaticum seedlings from the 12 areas was evaluated, and the results showed that the drought resistance at the sites went from strong to weak in this order: Xietongmen > Linzhou > Sog > Damxung > Tingri > Namling > Gyirong > Linzhi > Purang > Dingjie > Longzi > Sa’gya. The drought resistance of P.centrasiaticum was strong in Xietongmen, Linzhou and Sog. Whether P.centrasiaticum from these three areas is suitable for cultivation in arid and semi-arid areas of Tibet needs further study.

Key words: Tibet, P.centrasiaticum Tzvel., seedling stage, physiological characteristics, drought resistance

ZHANG Guangyu, WANG Jiangwei, ZHANG Haorui, FU Gang, SHEN Zhenxi. Comparative Study of the Impact of Drought Stress on P.centrasiaticum at the Seedling Stage in Tibet[J]. Journal of Resources and Ecology, 2020, 11(3): 322-328.

Figures/Tables 5

References 37

1	Abrams M, Kubiske M, Steiner K . 1990. Drought adaptations and responses in five genotypes of Fraxinus pennsylvanica Marsh: Photosynthesis, water relations and leaf morphology. Tree physiology, 6(3):305-315.
2	Cui G W, Li B, Wang M J , et al. 2015. Development status existing problems and their solutions of artificial pasture in Tibet. Heilongjiang Animal Science and Veterinary Medicine, 11(21):137-138. (in Chinese)
3	Ding Y M, Ma L H, Zhou X G , et al. 2013. Effects of drought stress on free proline and malonaldedyde contents in potato eaves and correlation analysis of drought-tolerant level among different varieties. Southwest China Journal of Agricultural Sciences, 26(1):106-110. (in Chinese)
4	Duan C, Shi P L, Zhang X Z , et al. 2019. Suitability analysis for sown pasture planning in an alpine rangeland of the northern Tibetan Platea. Acta Ecologica Sinica, 39(15):5517-5526. (in Chinese)
5	Fu G, Shen Z X, Zhang X Z . 2018. Increased precipitation has stronger effects on plant production of an alpine meadow than does experimental warming in the Northern Tibetan Plateau. Agricultural and Forest Meteorology, 249:11-21.
6	Gao F X . 2008. The investigation of native ground-cover plant and research of ecological and biologicaal characteristics on P. centrasiaticum. PhD diss., Lanzhou: University of Lanzhou.
7	Gao Q Z, Li Y, Xu H M , et al. 2014. Adaptation strategies of climate variability impacts on alpine grassland ecosystems in Tibetan Plateau. Mitigation and Adaptation Strategies for Global Change, 19(2):199-209.
8	Hegarty T . 1977. Seed activation and seed germination under moisture stress. New Phytologist, 78(2):349-359.
9	Jiang Y, Huang B . 2001. Drought and heat stress injury to two cool-season turfgrasses in relation to antioxidant metabolism and lipid peroxidation. Crop science, 41(2):436-442.
10	Li H S. 2000. Principles and techniques of plant physiological biochemical experiment. Beijing: Higher Education Press. (in Chinese)
11	Li T Y . 1983. Study on domestication and cultivation of P. centrasiaticum. Prataculture & Animal Husbandry, ( 2):55-57. (in Chinese)
12	Li S, Yao Z, Liu Z , et al. 2019. The spatio-temporal characteristics of drought across Tibet, China: Derived from meteorological and agricultural drought indexes. Theoretical and Applied Climatology, 137(3-4):2409-2424.
13	Liao L, Wu J S, Wu J , et al. 2018. Effects of drought stress on the growth indexes and physiological biochemical characters in leaves of osmanthus fragrans seedling. Journal of West China Forestry Science, 47(2):54-58. (in Chinese)
14	Liu X Y, Long R J, Shang Z H , 2012. Interactive mechanism of service function of alpine rangeland ecosystems in Qinghai-Tibetan Plateau. Acta Ecologica Sinica, 32(24):7688-7697. (in Chinese)
15	Liu Z J, Zhang X L, Bai J G , et al. 2009. Exogenous paraquat changes antioxidant enzyme activities and lipid peroxidation in drought-stressed cucumber leaves. Scientia Horticulturae, 121(2):138-143.
16	Lutts S, Majerus V, Kinet J M . 1999. NaCl effects on proline metabolism in rice (Oryza sativa) seedlings. Physiologia Plantarum, 105(3):450-458.
17	Medrano H, Escalona J M, Bota J , et al. 2002. Regulation of photosynthesis of C3 plants in response to progressive drought: Stomatal conductance as a reference parameter. Annals of botany, 89(7):895-905.
18	Michel B E, Kaufmann M R . 1973. The osmotic potential of polyethylene glycol 6000. Plant Physiology, 51(5):914-916.
19	Mou F J, Chen L P, Li J P , et al. 2016. The dynamics of physiological and biochemical indexes of Keteleeria xerophila under drought stress. Journal of Arid Land Resources and Environment, 30(5):180-184. (in Chinese)
20	Munns R, Brady C J, Barlow E W R . 1979. Solute accumulation in the apex and leaves of wheat during water stress. Functional Plant Biology, 6(3):379-389.
21	Ni Y, Guo Y J, Lv J , et al. 2004. Physiological-biological changes of legumes under drought stress. Chinese Journal of Soil Science, 35(3):275-278. (in Chinese)
22	Pan X, Qiu Q, Li J Y , et al. 2014. Physiological indexes of six plant species from the Tibetan Plateau under drought stress. Acta Ecologica Sinica, 34(13):3558-3567. (in Chinese)
23	Singh T, Aspinall D, Paleg L . 1972. Proline accumulation and varietal adaptability to drought in barley: A potential metabolic measure of drought resistance. Nature New Biology, 236(67):188-190.
24	Sun H L, Zheng D, Yao T D , et al. 2012. Protection and construction of the National Ecological Security Shelter Zone on Tibetan plateau. Acta Geographica Sinica, 67(1):3-12. (in Chinese)
25	Van den Berg L, Zeng Y J . 2006. Response of South African indigenous grass species to drought stress induced by polyethylene glycol (PEG) 6000. South African Journal of Botany, 72(2):284-286.
26	Wang H Z, Xu Y L, Zhang C L , et al. 2015. Effects of drought stress on osmotic adjustment substances and antioxidant enzymes activity of Populus euphratica and Populus pruinosa seedlings. Journal of Arid Land Resources and Environment, 29(12):125-130. (in Chinese)
27	Wiggans D R, Singer J W, Moore K J , et al. 2012. Response of continuous maize with stover removal to living mulches. Agronomy Journal, 104(4):917-925.
28	Wong Y S, Luo G H, Kwan K M . 1997. Peroxidation damage of oxygen free radicals induced by cadmium to plant. Acta Botanica Sinica, 39(6):522-526. (in Chinese)
29	Wright S . 1969. An increase in the “inhibitor-β” content of detached wheat leaves following a period of wilting. Planta, 86(1):10-20.
30	Wu M, Zhang W H, Zhou J Y , et al. 2014. Effects of drought stress on growth, physiological and biochemical parameters in fine roots of Quercus variabilis BI. seedlings. Acta Ecologica Sinica, 34(15):4223-4233. (in Chinese)
31	Xie X Y, Zhang X, Zhang B . 2013. Evaluation of drought resistance and analysis of variation of relevant parameters at seedling stage of rapeseed (Brassica napus L). Scientia Agricultura Sinica, 46(3):476-485. (in Chinese)
32	Yan L L, Zhang Y, Bai C J . 2018. Comparative study on the production performance of 13 Stylosanthes guianensias. Pratacultural Science, 35(4):867-875. (in Chinese)
33	Zhang B W, Li F P, Wu Y L , et al. 2018. Drought resistance of five herbs under drought stress simulated by PEG-6000. Molecular Plant Breeding, 16(8):288-297. (in Chinese)
34	Zhang J, Kirkham M . 1994. Drought-stress-induced changes in activities of superoxide dismutase, catalase, and peroxidase in wheat species. Plant and Cell Physiology, 35(5):785-791.
35	Zhang M S, Xie B, Tan F , et al. 2003. Relationship among soluble protein, Chlorophyll and ATP in sweet potato under water stress with drought resistance. Scientia Agricultura Sinica, 36(1):13-16. (in Chinese)
36	Zhang X Z, He Y T, Sheng Z X , et al. 2015. Frontier of the ecological construction support the sustainable development in Tibet Autonomous Region. Bulletin of Chinese Academy of Sciences, 30(3):306-312. (in Chinese)
37	Zhou W W, Wang Y, Du J . 2009. Influences of drought stress on the physical characteristics of sedums species. Forest Research, 22(6):829-834. (in Chinese)

Source of seedlings	Geographical coordinates	Elevation (m)	Compact form
Linzhi	93° 94′ N, 29° 79′ E	3117	LZS
Linzhou	91° 08′ N, 29° 88′ E	3889	LZX
Xietongmen	88° 21′ N, 29° 42′ E	3891	XTM
Sog	93° 79′ N, 31° 83′ E	3940	SX
Longzi	92° 32′ N, 28° 42′ E	3948	LZ
Namling	89° 08′ N, 29° 64′ E	3949	NML
Purang	81° 16′ N, 30° 34′ E	4061	PL
Dingjie	87° 77′ N, 28° 37′ E	4163	DJ
Gyirong	85° 32′ N, 28° 89′ E	4198	JL
Sa’gya	87° 90′ N, 29° 00′ E	4233	SJ
Damxung	91° 05′ N, 30° 51′ E	4333	DX
Tingri	87° 04′ N, 28° 47′ E	4413	DR

Source of seedlings	Geographical coordinates	Elevation (m)	Compact form
Linzhi	93° 94′ N, 29° 79′ E	3117	LZS
Linzhou	91° 08′ N, 29° 88′ E	3889	LZX
Xietongmen	88° 21′ N, 29° 42′ E	3891	XTM
Sog	93° 79′ N, 31° 83′ E	3940	SX
Longzi	92° 32′ N, 28° 42′ E	3948	LZ
Namling	89° 08′ N, 29° 64′ E	3949	NML
Purang	81° 16′ N, 30° 34′ E	4061	PL
Dingjie	87° 77′ N, 28° 37′ E	4163	DJ
Gyirong	85° 32′ N, 28° 89′ E	4198	JL
Sa’gya	87° 90′ N, 29° 00′ E	4233	SJ
Damxung	91° 05′ N, 30° 51′ E	4333	DX
Tingri	87° 04′ N, 28° 47′ E	4413	DR

Sites	MDA	Pro	Chlorophyll	Average membership function value	Ranking
Linzhi	0.341	0.746	0.232	0.440	8
Linzhou	0.460	0.768	0.515	0.581	2
Sog	0.505	0.379	0.662	0.515	3
Xietongmen	0.318	0.834	0.637	0.596	1
Longzi	0.714	0.326	0.165	0.402	11
Namling	0.737	0.398	0.207	0.448	6
Purang	0.515	0.438	0.366	0.440	9
Dingjie	0.647	0.390	0.263	0.433	10
Gyirong	0.729	0.229	0.372	0.443	7
Sa’gya	0.651	0.296	0.178	0.375	12
Damxung	0.680	0.407	0.302	0.463	4
Tingri	0.740	0.340	0.289	0.456	5

Sites	MDA	Pro	Chlorophyll	Average membership function value	Ranking
Linzhi	0.341	0.746	0.232	0.440	8
Linzhou	0.460	0.768	0.515	0.581	2
Sog	0.505	0.379	0.662	0.515	3
Xietongmen	0.318	0.834	0.637	0.596	1
Longzi	0.714	0.326	0.165	0.402	11
Namling	0.737	0.398	0.207	0.448	6
Purang	0.515	0.438	0.366	0.440	9
Dingjie	0.647	0.390	0.263	0.433	10
Gyirong	0.729	0.229	0.372	0.443	7
Sa’gya	0.651	0.296	0.178	0.375	12
Damxung	0.680	0.407	0.302	0.463	4
Tingri	0.740	0.340	0.289	0.456	5

[1]	Sydney M. GREENFIELD, Aliana C. NORRIS, Joseph P. LAMBERT, Wu liji, Se yongjun, ZHAN Jinqi, MA Bing, LI Deng, SHI Kun, Philip RIORDAN. Ungulate Mortality due to Fencing and Perceptions of Pasture Fences in Part of the Future Qilianshan National Park [J]. Journal of Resources and Ecology, 2021, 12(1): 99-109.
[2]	SHI Peili, ZHANG Xianzhou. Towards Regional Synergy: Reconciling Rangeland Ecological Functioning with Forage Production of Cultivated Pasture [J]. Journal of Resources and Ecology, 2020, 11(3): 247-252.
[3]	NIU Ben, HE Yongtao, ZHANG Xianzhou, SHI Peili, DU Mingyuan. Satellite-based Estimates of Canopy Photosynthetic Parameters for an Alpine Meadow in Northern [J]. Journal of Resources and Ecology, 2020, 11(3): 253-262.
[4]	CAO Yanan, ZHANG Xianzhou, NIU Ben, HE Yongtao. Comparison of Methods for Evaluating the Forage-livestock Balance of Alpine Grasslands on the Northern Tibetan Plateau [J]. Journal of Resources and Ecology, 2020, 11(3): 272-282.
[5]	FENG Yunfei, DI Yingwei, ZHANG Jing, ZHANG Xianzhou, SHI Peili, Niu Ben. Impact of Grazing Exclusion on the Surface Heat Balance in North Tibet [J]. Journal of Resources and Ecology, 2020, 11(3): 283-289.
[6]	WANG Xiangtao, ZHANG Xianzhou, WANG Junhao, NIU Ben. Variations in the Drought Severity Index in Response to Climate Change on the Tibetan Plateau [J]. Journal of Resources and Ecology, 2020, 11(3): 304-314.
[7]	TIAN Yuan, ZHA Xinjie, GAO Xing, DAI Erfu, YU Chengqun. Spatial Analysis and Biogeochemical Cycles: A Comparative Study of Kashin-Beck Disease Villages and Non-disease Villages in Linzhou County, Tibet [J]. Journal of Resources and Ecology, 2020, 11(2): 232-246.
[8]	ZHOU Yuke. Characterizing the Spatio-temporal Dynamics and Variability in Climate Extremes over the Tibetan Plateau during 1960-2012 [J]. Journal of Resources and Ecology, 2019, 10(4): 397-414.
[9]	CUI Mingyue,WANG Junbang,WANG Shaoqiang,YAN Hao,LI Yingnian. Temporal and Spatial Distribution of Evapotranspiration and Its Influencing Factors on Qinghai-Tibet Plateau from 1982 to 2014 [J]. Journal of Resources and Ecology, 2019, 10(2): 213-224.
[10]	ZHANG Yang, LI Zhanbin, HAN Jichang, LI Peng, LI Juan. Effect of Covering-soil Thickness on Crop Growth on Bare Rock nd Gravel Land in an Ecological Restoration Project [J]. Journal of Resources and Ecology, 2018, 9(5): 484-492.
[11]	TIAN Li, ZHANG Yangjian, Claus HOLZAPFEL, HUANG Ke, CHEN Ning, TAO Jian, ZHU Juntao. Vegetation Pattern in Northern Tibet in Relation to Environmental and Geo-spatial Factors [J]. Journal of Resources and Ecology, 2018, 9(5): 526-537.
[12]	TIAN Yuan, YU Chengqun, ZHA Xinjie, WU Jianshuang, GAO Xing, FENG Chujian. Geographical and Botanical Variation in Concentrations of Molybdenum in Natural Pasture Plants and Surface Water and Yak Molybdenum Ingestion in North Tibet, China [J]. Journal of Resources and Ecology, 2018, 9(5): 545-553.
[13]	FU Gang, SUN Wei, LI Shaowei, ZHONG Zhiming. Response of Plant Growth and Biomass Accumulation to Short-term Experimental Warming in a Highland Barley System of the Tibet [J]. Journal of Resources and Ecology, 2018, 9(2): 203-208.
[14]	LI Wenhua. An Overview of Ecological Research Conducted on the Qinghai-Tibetan Plateau [J]. Journal of Resources and Ecology, 2017, 8(1): 1-4.
[15]	ZHANG Xianzhou, WANG Ling, HE Yongtao, DU Mingyuan, ZHANG Jing, SHI Peili, YU Chengqun, ZHANG Yangjian. Impact of Water Vapor on Elevation-dependent Climate Change [J]. Journal of Resources and Ecology, 2017, 8(1): 5-9.